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Thesis Name: Analysis of amino acids in medicine by micellar electrokinetic capillary with high frequency conductivity detection
Usage: medical chemistry
Keyword: micellar electrokinetic capillary chromatography, high frequency conductivity detection, amino acid,
Remarks: Abstract
The research about the separation and determination of amino acids is submitted. In the optimum separation condition, i, e, running buffer: 1mmol/LSodium Lauryl Sulfate(SDS), 7.5mmol/L sodium dihydrogen phosphat,5mmol/L formic acid, (pH3.8) and the separation voltage 20.5 kV, the baseline separation was obtained in six mines for the five amino acids, phenylalanine, arginine, alanine, glutamic acid, aspartic acid, and the separation efficiency was between 0.72×105and 2.48×105N/m.The high frequency conductivity detection was used in this separation, and its detection mode is a kind of indirect measurement. The more the difference between the buffer and the sample is,the more the detection sensitivity is. In the optimum detection condition, the detection limit for the phenylalanine, arginine, alanine, glutamic acid, aspartic acid, was 0.03,0.63,0.74,2.01and 0.92mmol/L, respectively. This analytical method was used to determination the amino acids in injection, and the analytical result was correspond with certificate.


Author:
Xiang Xiaolan,Yu Bin He Jinlan
Unit:
Department of Chemistry, Zhanjiang Normal College, Zhanjiang 524048;
College of Sciences, Nanjing University of Technology, Nanjing 21000
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